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Predissociated Dimers and Molten Globule Monomers in the Equilibrium Unfolding of Yeast Glutathione ReductaseLouzada, Paulo RobertoABSTRACTThe equilibrium unfolding of dimeric yeast glutathione reductase (GR) by guanidine hydrochloride (GdnHCl) was investigated
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For Trp residues in proteins, it is not uncommon to find average lifetimes that are significantly longer than for free Trp
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Since the frequency of the solvent-exposed secondary structures is smaller than the buried ones (44,45), the above sequential events could be interpreted as follows: the unfolding of the solvent-exposed β-sheet structures is the earliest event, followed by the formation of aggregates, and finally the unfolding of the other native structures
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5), 10 mM sodium pyruvate, 1 mM MgCl^sub 2^, followed by extensive dialysis against the same buffer in the absence of MgCl^sub 2^ and pyruvate
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When we examined BAL fluid from COPD patients, we found increased numbers of apoptotic cells and decreased numbers of apoptotic cell ingestions, which is consistent with an efferocytosis defect (W
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J Am Coll Cardiol 2006 (in press)(59) Asada K, Sasaki S, Suda T, et al
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For all heating rates the secondary structure elements (mainly α-helices) fully disappear and compared to GndHCl-induced unfolding (data not shown here) the CD signals are identical
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Hydrodynamic radii of native and denatured proteins measured by pulse field gradient NMR techniques
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Intracellular molecules have smaller diffusion coefficients than extracellular molecules because intracellular and compartmental boundaries restrict diffusion
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, 1999); however, there still exists some disagreement about exact location of monocations and distribution of bound monocations between different binding sites on DNA
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Vertical dimensions of the fibrillar aggregates were obtained from individual cross-section profiles generated by the AFM's accessory data analysis software
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2]), was estimated from swept area
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For further simulation details, we refer to Hsu and Bonvin (2004)
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12 µm, but the particles tend to agglomerate
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curriculum has not been reported
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Total inventory scores were used in this study
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