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. 1-cm path length cuvette on a Jasco spectropolarimeter 715 (Jasco, Tokyo, Japan) attached to a Peltier PTC-348 WI
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. quantifying the interactions between unfolded molecules at high concentrations (29), assessing the presence of residual interactions between hydrophobic as well as charged groups (119-122)
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. Hence, there are four hinge regions in the CaM dimer
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. In steady shear, the zero shear viscosity of concentrated solutions at pH 5
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. To reduce the possibility of tip-contamination, samples were engaged at a zero scan position
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. This feature of FRET has been used extensively to study the structure and dynamics of biological molecules and assemblies, in ensemble as well as more recent single-molecule measurements (Selvin 2000; Klostermeier and Millar, 2001b; Weiss, 2000; Ha, 2001)
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. Journal of Personality and Social Psychology, 32(6), 951-955
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