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Thermal unfolding transitions were monitored by taking the CD signal at 222 nm as a function of temperature
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5 × 10^sup -11^ m^sup 2^s^sup -1^, in agreement with previous measurements carried out under similar conditions and concentration (33)
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This suggests that the protein refolding detected by CD between 32 and 24°C upon relatively rapid cooling (Fig
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5 mL Freund's complete adjuvant (Nacalai Tesque, Inc
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. The final enzyme concentration for the activity assay was 2 µM (~0
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. In this collection of 11 articles, contributors describe their work in PTKs in such areas as using PTKs as targets for cancer and other indications, targeting Src homology 2 domains in drug discovery, using P12-kinase inhibition as a target for therapeutic intervention, using an activated FLT3 receptor tyrosine kinase as a therapeutic target in leukemia and JAK kinases in leukemia, lymphomas and multiple myeloma, applying platelet-derived growth factor, using a targeted therapy for myelogenous leukemia, testing signal transduction inhibitors in animal models of cancer, and using phosphoproteomics in drug discovery and development
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