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5 C to avoid the possible artifacts caused by large band shifts accompanied with slight intensity changes in this temperature range (see also Fig
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Molecular graphics were created with PyMOL (38)
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(37) This may be especially important in suppressing protease/antiprotease imbalance during acute inflammation
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In supercooled solutions ANS binds with a dissociation constant in the µM range (~5 µM) and no further intensity increment beyond the control is observed up to 450 µM, practically the maximum ANS concentration before inner filter artifacts and declining sensitivity become prohibitive
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The use of small integration areas has the advantage that the noise is still averaged, while at the same time partially overlapping crosspeaks still can be reliably integrated (21)
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Importantly, all probes show different kinetics, with varying amplitudes and rates during the reaction (Table 1)
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Pressure measurements were performed using a small quartz bottle with a lid that collapses under pressure
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This close correlation of binding and activation of these two species which have such different affinities suggests that the change in emission intensity correlates to activation
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Incubation of the heat-denatured samples at 24°C leads to gradual disk reconstitution accompanied by a reduction in the amount of lipid-poor apoA-2 ( Fig
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7 mm, were used to describe larval development
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The smallest larva collected (yolksac larva) was 3
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Identification of formalin-preserved eggs of red sea bream using monoclonal antibodiesShingo HiroishiCatches of important commercial fish such as red sea bream, flat fish, and yellowtail are decreasing in Japan
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In contrast, our results imply the presence of a folding intermediate with the two arms in close enough proximity to allow formation of specific loop-loop contacts
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- Implications for reconstitution of the active enzyme
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