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That this is indeed the case is shown by comparing cavity statistics in the EV limit to values obtained for freely rotating chains application make professional up .
5 the protein conformation undergoes a reversible transition which leads to a swelling of the monomeric unit (29,30) application make professional up .
Native tetrameric TTR can serve as a reference state to which some, but not all, fluorescence probes used in this study can bind application make professional up .
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The consequent mutual but general attraction of the helices for each other permits the final diffusive search for the native state to take place in a two-dimensional space, rather than the full five-dimensional search configuration space of all the diffusive degrees of freedom available to the protein once the secondary structure has formed application make professional up .
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The FRET efficiency histogram of the GnI variant also exhibits two distinct subpopulations, with mean E values that are similar to the wild-type, but the amplitude of the high FRET peak is substantially reduced relative to the low FRET peak (Fig application make professional up .
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