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. MATERIALS AND METHODSMaterialsGR (type IV, from Baker's yeast), GSSG, NADPH, DTNB, GdnHCl, and HEPES were from Sigma Chemical (St
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. , 1995), and a single mutation is sufficient to prevent dimerization of human GR (Nordhoff et al, 1993)
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. Protein dynamics, folding and misfolding: from basic physical chemistry to human conformational diseases
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. Pleiotropic effects are the multiple, often seemingly unrelated characters caused by a single gene
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. Conversely, zooming out or scaling up from the contact density map of a short protein like PSBD will yield the contact density maps of longer proteins such as ubiquitin or titin
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. 12 support the conclusion that EV limit ensembles are topologically equivalent
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. coli, EIN^sup ec^ (9), and the C-terminal domain of T
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. The first step in sugar transport: crystal structure of the amino terminal domain of enzyme I of the E
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. 5 c, and as known for all α-amylases studied here, the protein concentration has no effect on the transition temperatures (17)
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. As for azurin (12), lowering the freezing temperature from -5°C to -13°C increases the number of binding sites but the average affinity is lowered
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. Aliquots of the aggregation reaction were withdrawn and mixed with an assay buffer (10 mM HCl, containing a final concentration of ANS, Bis-ANS, or DCVJ at 2
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. These studies have provided thermodynamic information on the global folding of the hairpin ribozyme (Klostermeier and Millar, 2000) and also quantified the energetic contributions of individual tertiary interactions (Klostermeier and Millar, 2001a, 2002)
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. Reaction was started by addition of 1
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. It is worth noting that the peak centered around 1639 cm^sup -1^ at temperatures above 80°C in the difference absorption spectra (Fig
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. Flory showed that a quantity such as the average radius of gyration (R^sub g^) will scale with chain length (N) according to a power law of the form R^sub g^ = R^sub o^N^sup ν^ (45)
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. For PPA, which already exposes tryptophan residues to the protein surface in the folded state, a blue shift of the emission spectrum occurs upon thermal unfolding (see Fig
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. Activity of the native batch (258 U/mg) was not significantly reduced by the neutron scattering experiments
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.Influence of helical junction geometry on docking efficiencyWe obtained FRET efficiency histograms for three ribozyme constructs with different helical junction geometries (natural 4WJ as well as corresponding 2WJ and 3WJ constructs; Fig
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