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Decomposition of protein tryptophan fluorescence spectra into log-normal components
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For all three fluxes shown in Fig
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2) and τ^sup o^^sub 1^ of 12
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The Θ^sub 222^(t) data show slow unfolding on a timescale of 10^sup 2^-10^sup 3^ s (as compared to 10^sup -8^-10^sup -10^ s for the helix-to-coil transition in solution; Yang and Gruebele, 2003), implicating high free-energy barrier ΔG*
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Taken together, the smooth E shifts with changing Mg^sup 2+^ concentration combined with the observed broadening/ tailing at high Mg^sup 2+^ concentration are consistent with the presence of a dynamic equilibrium between the quasi-docked state and an extended undocked state, with a relatively small energy barrier for interconversion (Fig
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