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. Van der Broeck, M
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. 0% glucosamine) of the mass of the lectin (16
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. Cavity statistics, P^sub oa^(r) folded ubiquitin are shown in Fig
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. 2 K; 4 µM, T^sub m^ = 329
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. Azodicarboxylic acid (diamide) was used to oxidize the cysteines to form the disulfide bridge and was present in all NMR samples
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. From the estimated maximum and minimum values of (τ^sub o^^sub m^)^sub app^, 12
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. For example, sodium ion, which has been reported to have the lowest DNA binding potential, is the most effective monocation for DNA compaction
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. The A-state also shows an increase in β-sheet structure over time in a slow process suggestive of structure formation during self-assembly
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. The lower value for the Bis-ANS case is (at least) partially due to the shorter lifetime that will truncate the measurement since the rotational correlation time is considerably longer than the decay lifetime
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. Shobini Jayaraman, Donald L
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. Cheese also is rich in branched chain amino acids (BCAA), known to play a role in insulin regulation and glucose sparing
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. Spent females were first collected during the last week of January and were most frequently collected in late February and early March
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. Monoclonal antibodies were developed according to the methods of Kohler and Milstcin (1975), Garfre and Milstein (1981), and Hiroishi et al
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. 10 This is demonstrated by the production of type-I collagen as opposed to type-II collagen which is characteristic of hyaline cartilage
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. 00J Bone Joint Surg [Br] 2005;87-B:128-34
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. Furthermore, of these 188 proteins, 104 contain both forms of glycosylation (6)
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. Snapshots were saved for analysis once every 10^sup 3^ trial moves
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. 8 B shows that it is in fact impossible to find room for small or large cavities unless the cavity is located sufficiently far from the center of mass of the folded protein
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. The transitions measured by CD became broader as the concentration increased, and although a small increase was observed when the concentration was raised (3 µM yielded T^sub m^ = 328
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. These calreticulin/PS-rich patches distribute away from regions rich in CD47 (a newly described "don't-eat-me" signal on viable cells), (11,17,18) ultimately resulting in enhanced effrocytosis
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. NMR and Its Applications to Living Systems
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. In particular, the monocation with the highest compaction activity (Na+) generated compact DNA at half the concentration than the monocation with the lowest potential (Cs+)
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. In a study that focused on the interaction between PLCβ^sub 2^- and Gβγ-subunits, we found, not surprisingly, that the measured steady-state dissociation constant for the two proteins was equal to the ratio of the on- and off-rates (Runnels and Scarlata, 1998)
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. 1, A and B), confirm that cold denaturation is an intrinsic property of the marginally stable S-S linked apoA-2 dimer
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. Additional funding was provided through Hatfield Marine Science Center scholarships: the 1996 Barbara Schwantes Memorial Fellowship; the 1997 Mamie L
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. Therefore, we developed monoclonal antibodies that allow P
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. American Journal of Psychiatry, 144, 1184-1188
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