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Accurate models for denatured states also impact a range of areas, including quantitative studies of in vitro folding pathways (10,19-24), protein design (25,26), studies of protein aggregation (27-29), and understanding preferential interactions in cosolute mixtures (1,3,30-36) barber comment irvin michael tiki .
Folding rates for two-state proteins show statistically significant correlation with native-state contact order (104) barber comment irvin michael tiki .
1 ) reach a more buried and nonpolar microenvironment in the unfolded state barber comment irvin michael tiki .
The preparation of the CaM:SEF2-1mp NMR samples has been described by us earlier (11) barber comment irvin michael tiki .
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Disk formation was confirmed by negative staining electron microscopy (EM) and non-denaturing gel electrophoresis which showed no free protein barber comment irvin michael tiki .
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