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Backbone Dynamics of a Symmetric Calmodulin Dimer in Complex with the Calmodulin-Binding Domain of the Basic-Helix-Loop-Helix Transcription Factor SEF2-1/E2-2: A Highly Dynamic ComplexLarsson, GöranABSTRACTCalmodulin (CaM) interacts specifically as a dimer with some dimeric basic-Helix-Loop-Helix (bHLH) transcription factors via a novel high affinity binding mode barber cut evanston,evanston hair .
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Structural interpretation of the nanosecond-timescale internal motionThe dimeric CaM:SEF2-1mp complex can in principle show internal modes of motion involving either the individual N- and C- terminal domains and/or the CaM monomers as a whole barber cut evanston,evanston hair .
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Time-resolved fluorescenceTime-resolved measurements of tryptophan (Trp) fluorescence were recorded using an IBH (Glasgow, UK) 5000 U fluorescence lifetime spectrometer system with 1 nm resolved excitation and emission monochromators (5000 M) barber cut evanston,evanston hair .
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A complementary approach is to characterize the conformational distributions of freely diffusing RNA molecules using a single-molecule method referred to as diffusion spFRET (Deniz et al barber cut evanston,evanston hair .
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