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. Among various methods, such as calorimetry, infrared spectroscopy, osmotic pressure analysis, dielectric spectroscopy, and nuclear magnetic resonance techniques, we used dielectric spectroscopy to study the hydration states of N, A, and U^sub A^ of apomyoglobin
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. The original IR or fluorescence data were normalised by the corresponding intensity of the spectra recorded al 30°C
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. Backbone Dynamics of a Symmetric Calmodulin Dimer in Complex with the Calmodulin-Binding Domain of the Basic-Helix-Loop-Helix Transcription Factor SEF2-1/E2-2: A Highly Dynamic ComplexLarsson, GöranABSTRACTCalmodulin (CaM) interacts specifically as a dimer with some dimeric basic-Helix-Loop-Helix (bHLH) transcription factors via a novel high affinity binding mode
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. Hence, the ratio f can be used to estimate the energy barrier between the exchanging states
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. Structural interpretation of the nanosecond-timescale internal motionThe dimeric CaM:SEF2-1mp complex can in principle show internal modes of motion involving either the individual N- and C- terminal domains and/or the CaM monomers as a whole
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. Time-resolved fluorescenceTime-resolved measurements of tryptophan (Trp) fluorescence were recorded using an IBH (Glasgow, UK) 5000 U fluorescence lifetime spectrometer system with 1 nm resolved excitation and emission monochromators (5000 M)
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. A complementary approach is to characterize the conformational distributions of freely diffusing RNA molecules using a single-molecule method referred to as diffusion spFRET (Deniz et al
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. To test whether there is a relationship between the extent of ANS binding induced by freezing and the instability of azurin to the freeze-thawing process, we have determined the degree of irreversible denaturation of WT and mutant species when subjected to repeated freeze-thawing cycles
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