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Iodide is a negatively charged quencher expected to quench the fluorescence emission of solvent-exposed tryptophan residues (Lakowicz, 1983)
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As for specific predictions, a polymer in the EV limit is best described in terms of two distinct length scales (39)
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10 we show distributions of distances obtained for different pairs of residues in the three proteins: PSBD, ubiquitin, and titin
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Conventional NMR experiments based on the nuclear Overhauscr effect are incapable of resolving contacts that go beyond 5-7 Å
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CONCLUSIONS We have used an atomistic EV model, developed in previous work, to show that it is computationally tractable to generate accurate conformational ensembles for proteins in the EV limit
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The results of the trypsin digestion and quenching experiments qualitatively agree, indicating that EP suffers conlbrmational changes upon binding to PEP
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Structural and dynamical features contributing to thermostability in alpha-amylases
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1 ppm, and the aceturate peaks (a, d, and e) at 1
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Our experiments are consistent with soman-induced stabilization resulting from an elevation of the free energy of the unfolded state, which arises mainly from a decrease in its entropy due to the covalent modification by soman
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In addition, using the simple general equation, which covers for both fast and slow exchange, derived by Ishima and Torchia (34), we also show that the exchange rate can be determined from a combination of CPMG measured R^sub ex^ and change in R^sub ex^, (ΔR^sub ex^, vide infra) without prior assumption on the exchange timescale
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However, the present data cannot exclude internal motion involving reorientation of the CaM monomers, which have an excepted timescale of ~7-9 ns
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