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. Although there were changes in the tertiary structure, EI^sup sc^ retained some, if not all, of its secondary structure, as shown by the increase (in absolute value) of the [Θ] at 222 nm (Fig
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. We note here that although we have interpreted peak shifts and broadening in terms of distance changes and ribozyme subpopulations, respectively, peak shifts could also potentially be caused by solvent effects on dye photophysics, and corresponding changes in the correction factor [gamma] (see Materials and Methods), as well as peak broadening by effects of [kappa]^sup 2^, the orientation factor (Deniz et al
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