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In addition, size-exclusion chromatography analysis indicated that the intermediates populated at 2
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This suggests that the association of hydrophobic moieties might follow the disruption of secondary structures from N to A states
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7 K when both effectors were present)
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Therefore, in this case, R-type flux having solder-flux interfacial tension 445 mN/mm (which is higher than the other two fluxes) contributes to the lower values of γ^sub LF^ - γ^sub SL^ and is attributed to the higher contact angles and negative wetting forces (Fig
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The CaM:SEF2-1mp complex is found to be highly flexible with internal motions on the picosecond, nanosecond, and microsecond timescales
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The slopes of the Arrhenius plots suggest activation energy E^sub a,fast^ = 22 ± 5 kcal/mol for the fast phase and E^sub a,slow^ = 60 ± 7 kcal/mol for the slow phase
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, 2000), and this remains in accordance with a threedimensional model proposed for insulin amyloid fibrils (Jimenez et al
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1) nested within the full high-dimensional search space
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The only other sympatric species of Pinguipedidae in the Argentine shelf is the Brazilian sandperch (Pinguipes brasilianus), which shares several similarities in meristic counts with P
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Thermal Stability With MQLWhen employing MQL, the strategies for maintaining thermal stability and part tolerances include minimizing the amount of heat introduced and compensating for thermal growth
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[beta]0+[beta]1FL/1 + [e
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The broad asymmetric peak shape observed for the SL 4WJ implies that extended undocked and quasi-docked states are in rapid conformational exchange, and our kinetic simulations indicate that the rate constant for formation of the quasi-docked state is in the range 10-41 × 10^sup 3^ s^sup -1^, whereas the corresponding reverse rate constant is in the range 3
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Energetics of hydrogen bond networks in RNA: Hydrogen bonds surrounding G+1 and U42 are the major determinants for the tertiary structure stability of the hairpin ribozyme
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Journal of Nursing Education
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. Taken together, these results suggest that at least two intermediate states can be identified in the equilibrium unfolding of GR, namely a partially unfolded predissociated state and a molten globule monomer
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. It is worth noting that the peak centered around 1639 cm^sup -1^ at temperatures above 80°C in the difference absorption spectra (Fig
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. The main point of the foregoing discussion is that inasmuch as there is congruence between the EV-limit ensemble and highly denatured states, chain fluctuations create ample room to accommodate favorable interactions with the surrounding solvent
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. In EI^sup sc^, a single peak was observed in CD and DSC experiments, but in the pH range where the thermal transitions could be followed (pH 7-9), two different peaks were not observed; further, the T^sub m^ of the sole transition observed was pH-indcpendent between pH 7 and 9 (Fig
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. NMR characterization of the dynamics of biomacromolecules
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. It is insensitive to small variations in ^sup 15^N chemical shift anisotropy (CSA) (from -150 to -200 ppm) (22)
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.Model selection, order parameters, and timescales for internal motionBased on the qualitative analysis of the RT^sup n^^sub 1^ versus NOE plots, Fig
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. Interfacial rheology of food emulsifiers and proteins
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.EXPERIMENTAL PROCEDURESProtein purificationTTR was expressed in BL21/DE3 cells transformed with the pmmHa plasmid
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. By comparing the volume change of PLCβ-Gβγ alone and in the presence of Gα^sub s^(GDP), we can estimate the volume of activation of PLCβ^sub 2^ by Gβγ-subunits
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.Surprisingly, the rank order for the disk stability indicated by our DSC, CD, and light-scattering data (Figs
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