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Runs were carried out at 23°C on a Shimadzu (Kyoto, Japan) LC-10AS HPLC at a flow rate of 0
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Transient association of newly synthesized unfolded proteins with the heat-shock GroEL protein
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Cornell University Press, Ithaca, NY
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This could be due to the fact that CD is reporting global changes in the secondary structure, and fluorescence is monitoring the changes in tertiary structure around fluorescent residues
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Besides the disappearance of secondary structure elements, in most cases the three-dimensional structures are substantially altered
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The remaining residues (see Table 1) show a weak decrease in exchange rate upon change in temperature (f [asymptotically =] 0
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de Beer, and D
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Larval development of the Pacific sandperch (Prolatilus jugularis) (Pisces: Pinguipedidae) from the Independencia Bight, Pisco, Peru
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, 2000; Russell et al, 2002a; Zhuang et al, 2002)
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In contrast, the specifically-docked conformation (exemplified by the WT 4WJ) is stabilized by an extensive hydrogen bond network (14 hydrogen bonds in all (Rupert and Ferre-D'Amare, 2001)), prolonging the lifetime of this state
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Immunolocalisation showed extensive staining for type-II collagen in pellets cultured in DMEM/ITS+/TGF-[beta]1 (Fig
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Only about 13 million Americans are currently taking statins, but federal guidelines suggest 23 million more should be on a similar regime
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Prescribing sight unseen and without follow-up monitoring "is not a valid veterinarian-client-patient relationship," she says
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If you purchase preventives on the Internet, "nobody stands behind them--the veterinarian doesn't, the manufacturer doesn't
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And that says nothing for Food and Drug Administration regulators who have given up plenty of skin in this scrap, too
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