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14), also known as Acylase I (ACYI), is a homodimeric enzyme containing one zinc ion per subunit (4-6)
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Here, we compare the ANS binding capacity of the four azurin species to inquire how these modified features modulate the ice-induced perturbation of tertiary structure
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This work was supported by grants from the Swedish Research Council (S
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The aggregation process produced fairly monodisperse oligomers (300-500 kD) within 2 h that matured after 20 h into larger spherical clusters (30-50 nm in diameter) and protofibrils as shown by transmission electron microscopy
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2, B and C) than in similar complexes with apoA-1 (data not shown)
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