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. Soybean agglutinin (gSBA) is a tetrameric glycoprotein with a subunit molecular mass of 29,495 Da
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. REFERENCES L Rudd, P
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. The folding features of both domains are different
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. MATERIALS AND METHODS Enzymes α-Amylase from Bacillus licheniformis (BLA; purchased from Sigma, Taufkirchen, Germany), from Bacillus amyloliquefaciens (BAA; purchased from Fluka, Buchs SG, Switzerland), from Bacillus subtilis (BSUA; from Fluka)
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. Whereas the results at pH 5
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. The "bare" BLG dimer may be approximated to a prolate ellipsoid with a length of 6
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. The results of DNA compaction by metal chlorides in PEG clarify that in some cases the orders of relative binding and compaction activities can be even opposite
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. These folding intermediates exhibited flexible tertiary structures and nativelike secondary structures
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. In this case, deglycosylation lowers the T^sub m^ of the peptide by 20°C, whereas the free energy of unfolding is reduced by only 1 kcal/mol (26)
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