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CD measurements in the far-ultraviolet (UV) region Spectra were collected on a Jasco J810 (Tokyo, Japan) spectropolarimeter fitted with a thermostated cell holder and interfaced with a Peltier cell, working at 298 K
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Spatial localization in NMR spectroscopy in vivo
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Cavities in proteins: structure of a metmyoglobin-xenon complex solved to 1
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020 [Angstrom], and the ^sup 15^N CSA value to -170 ppm
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We also employed the classic and widely used, although poorly understood, amyloid fluorescence dye ThT, which is known to interact with amyloid fibrils (Naiki et al
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Although we predict similar volume changes for this association in the two different environments, the apparent affinities will differ because the effective concentration of membrane-bound proteins tends to be much higher due to their confinement on the reduced area of the membrane surface giving stronger apparent affinities (see Runnels and Scarlata, 1999)
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After 48-h incubation, the disk reconstitution appears incomplete and a sharp lipid transition of reduced amplitude is detected (Fig
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This is because the rate-determining diffusive searches will in general be completed only when a topological, as well as a spatial, constraint in the final native state is satisfied for the first time
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(See Reproducible 2 for details
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--Pharmaceutical Sciences* WASHINGTONUniversity of Washington School of Pharmacy MS
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* Soft Hand--A hand with an ace, which can be counted as 11
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Such events may be irrelevant in the eyes of the researcher, but they can affect the decision maker's estimation of the likelihood of various outcomes, the decision, and the confidence in the correctness of the decision
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