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. The transitions measured by CD became broader as the concentration increased, and although a small increase was observed when the concentration was raised (3 µM yielded T^sub m^ = 328
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. In a second experiment, we compared dimeric DmAChE and tetrameric HuBChE
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. Above that temperature, the MSDs for HuBChE-D^sub 2^O are larger than those for Soman/HuBChE-D^sub 2^O, which remain in a quasiharmonic regime, as shown by their linear dependence on temperature (Fig
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. Note that potential variation of the ^sup 15^N CSA between -150 and -200 ppm hardly affects RT^sup n^^sub 1^ (±3%), and thus does not affect the conclusions (22)
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. of Physical Chemistry-Laboratory of Biophysical Chemistry, University of Nijmegen, Toernooiveld 1, 6525 ED, Nijmegen, The Netherlands
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. Osmotic pressure and viscoelastic shear moduli of concentrated emulsions
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. Initiation of oligomerization was performed through addition of 100 mM NaCl from a 4 M stock solution to 0
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. Several amyloidogenic in vitro assays employ acidic pH for generation of amyloid fibrils; however, ThT shows very modest fluorescence at acidic pH when bound to protofibrillar amyloid of TTR (Fig
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. Furthermore, information on the effect on folding rate of replacing specific residues via mutation or "Φ-value" analysis (Fersht, 2000) needs to be taken together with correlations of contact order
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. The simulation also found that kinetic properties are more closely connected with Φ than local "degree of nativeness"
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. Interestingly, for CD4-F43, the corresponding value is negative, -0
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. Subsequently, the native tertiary structure is formed through a slow (rate-limiting) conformational search and/or consolidation of specific metal-ion binding sites
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.2 M^sup -1^) were added
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. Evolutionary conservation and variation of protein folding pathways
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. According to the simple ω^sup 7/4^ law proposed by Hoult (50), a SNR increase by 50% is expected between 14
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. Thus, the data are consistent with a motional model in which the two N-terminal and two C-terminal CaM domains in the dimeric CaM:SEF2-lmp complex undergo a small-scale wobbling motion with a half-angle of ~20° as estimated from S^sup 2^^sub s^ = 0
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.23 kJ K^sup -1^ mol^sup -1^
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. Hence, the helical junction geometry strongly affects the tertiary folding of the hairpin ribozyme
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