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With that, a thunderous holler went up
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Solvent-accessibility of aromatic residues (acrylamide quenching experiments) Quenching experiments in the presence of the effectors yielded quenching constants in the presence of Mg^sup 2+^ (2
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The results of the trypsin digestion and quenching experiments qualitatively agree, indicating that EP suffers conlbrmational changes upon binding to PEP
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0 A resolution: mechanistic implications
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Although the wraparound mode of target binding is the most studied, alternative binding modes have become evident
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Azodicarboxylic acid (diamide) was used to oxidize the cysteines to form the disulfide bridge and was present in all NMR samples
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Given the anisotropy and the maximum (τ^sup o^^sub m^), τ^sup o^^sub 1^, the angle Φ that each N-H^sup N^ bond vector makes with the main axis of the diffusion tensor can be calculated
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In conclusion, the CaM:SEF2-1mp binding seems indeed distinct from other known protein:target binding modes
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The conformational sampling requires more time than for CD4
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Likewise, the Mg-binding parameters (K^sup Mg^^sub D^ and n) obtained from the previous trFRET data are also in reasonable agreement with the corresponding values reported here
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Additionally, the quasi-docked species appears to form very rapidly (see below), suggesting that this species could act as a kinetic intermediate in the docking pathway of the natural ribozyme
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MOHLENBROCK is professor emeritus of plant biology at Southern Illinois University in Carbondale
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