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. Thus the intrinsic Trp fluorescence was used to investigate the changes of the tertiary structure of ACYI during thermal denaturation
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. Each measurement was an average of three readings
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. Crystal structure analysis of oxidized Pseudomonas aeruginosa azurin at pH 5
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. To examine the reason for this factor, we compared T^sub 2^, the spin-spin relaxation times, of the methylene peak at 1
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. Approximately half of the HuBChE was inhibited with soman, as described by Masson and Goasdoue (35), before dialysis and lyophilization
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. The CD melting curves at 222 nm, Θ^sub 222^(T), recorded during sample heating and cooling from 1 to 80°C, show that the helical structure reaches maximum at 25°C and completely unfolds upon heating as well as cooling from 25°C (Fig
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. AcknowledgmentsThis study resulted from cooperative efforts of the USGS Biological Survey Unit and the Department of Zoology, National Museum of Natural History
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.01 and R^sub o^ = 2
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. Protein concentration was 4 µM, unless otherwise indicated
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. In general, the presence of Mg^sup 2+^ +, PEP, or both effectors together had a minor effect on the thermal stability of the protein as judged by the slight changes in T^sub m^ (the ΔΔT^sub m^s from DSC measurements equal + 1
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