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INTRODUCTION Posttranslational modification is one of those events in biology that not only adorn a protein with variety but also impart it with different physicochemical properties business plan barber shop .
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Before comparing the behavior of individual proteins it is convenient to divide the group into two classes: one (β-gal, α-amylase, and ALD), which binds ANS essentially only in the frozen state, and the rest, which bind a fair amount of ANS also in liquid solution (4,5,28) business plan barber shop .
This trend is consistent with a pronounced binding capacity of oligomers whose subunits tend to dissociate in frozen aqueous media thereby making accessible large hydrophobic patches of the subunit interface business plan barber shop .
Self-diffusion and cooperative diffusion of globular proteins in solution business plan barber shop .
It is difficult to explain this inverse relationship between the compaction potentials of Na+ and K+ and their binding abilities business plan barber shop .
Fluorescence spectroscopy provides a very attractive alternative for such studies, due to sensitivity, ease of use, and flexibility business plan barber shop .
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Additionally, since the low E peak indicates a species with a longer inter-arm distance, we assign this peak to an extended (undocked) conformation business plan barber shop .
DISCUSSIONComparison of spFRET histograms and ensemble trFRET measurementsOur spFRET results for the wild-type ribozymes show the presence of extended (undocked) and docked conformations, with relative populations that are strongly dependent on the Mg^sup 2+^ concentration business plan barber shop .
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