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in communications from the New York Institute of Technology and an M
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Caves, in general, are food-poor environments, and cave fish have evolved a highly efficient metabolism that enables them to survive on fewer calories than their surface counterparts need
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MATERIALS AND METHODS Materials Tris (hydroxymethyl) aminomethane buffer, [N-[2-hydroxyethyl]piperazine-N'-[2-ethanesulphonic acid]] (HEPES), and guanidinium hydrochloride were from Sigma Chemical (St
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Although the average shape in the EV limit is that of a prolate ellipsoid, the fluctuations in the R^sub g^ and δ-values are large
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1 A) and the fluorescent residues were buried (Table 1)
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The rate determining step is not the rapid formation of local secondary structure, nor the diffusion of subdomains per se, but the organization of large pieces of secondary structure into the same topological configuration as the native state, which thereafter is able to form rapidly
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SEM images indicate that a continuous film forms at a curing time of 1 minute, but the film is not continuous after curing for 3 minutes
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Since the docked and undocked peaks are well resolved, any interconversion between the two conformers must occur more slowly than the ~280-µs average burst duration of the single-molecule measurements (see below)
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