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In the final structure, the interface domain is hidden in a hydrophobic, packed core surrounded by the other domains, as demonstrated by crystallographic studies of human GR (Schulz et al
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The heat flow versus temperature profile of ACYI contained an asymmetric positive peak arising from an exothermic transition (Fig
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This could be due to the fact that CD is reporting global changes in the secondary structure, and fluorescence is monitoring the changes in tertiary structure around fluorescent residues
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In EI^sup ec^, the thermal midpoint of the transition monitored by fluorescence is concentration-dependent (52), but the slopes of the native baseline are smaller due to the fact that two tryptophan residues are observed in the C-terminus of EI^sup ec^
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It may also be possible that a smaller degree of subunit dissociation in part explains the sharp increase in the residual enzymatic activity, retained after freeze-thawing, with increasing protein concentration (14,16,29)
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The fluorescence intensity of the different probes in the presence of the unfolded monomer state is shown in Fig
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The high-temperature transition in apoA-2: DMPC disks (solid circles, Fig
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Scavenger receptor class B, type I-mediated uptake of various lipids into cells
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We initially followed the gross maturity stage scheme of Nichol and Pikitch (1994) for darkblotched rockfish (Sebastes crameri) but ultimately abandoned their classification of maturity stages in favor of the simplified maturity stages reported by Gunderson et al
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The difference between our estimate and McClure's was most likely due to using whole otoliths to age fish, which resulted in underestimates of age, and to assigning maturity stages only during summer months, which we have already described as problematic
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Intracranial penetration due to BB air rifle injuries
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Pediatr Emerg Care 1990:6:278-9
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Bones and teeth were identified by comparison with museum specimens
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2b), containing clearly defined collagen fibrils (Figs 5b and 5c)
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Society and Leisure, 17, 189-212
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