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Interestingly, similar results are obtained for the protein modeled as a fully extended conformation (Fig
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Are proteins well packed? Biophys
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Contact order, transition slate placement, and the refolding rates of single domain proteins
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In particular the wavelength of the tryptophan fluorescence emission peak (λ^sub max^) is a valuable parameter to characterize properties of the unfolded state
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3 B emphasizes that the value both of n and of the apparent dissociation constant are considerably affected by temperature
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Data analysisConversion of the apparent K(d) obtained on membrane surfaces to the K(d) that would be obtained if the proteins were freely diffusing has been previously treated by assuming that the proteins are confined to interact within a reduced surface volume (ν) that is equal to the surface area of the membrane multiplied by the distance from the membrane surface into the aqueous phase where the proteins interact, which is taken to be 50 nm
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6 A, where the degree of dissociation was calculated as described in the methods
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