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. The samples were excited al 370 nm and emission scan was obtained from 420-520 nm
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. HuBChE-D^sub 2^O versus Soman/HuBChE-D^sub 2^OMSDs for the native and the inhibited enzyme are identical in the temperature range below 334 K (61°C) and increase smoothly (Fig
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. In one series of DSC experiments, the heat capacity C^sub p^(T) was recorded from the same sample during repetitive heating from 5 to 100°C followed by incubation at 24°C for 0-48 h (Fig
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. Larger proteins may do this in an increasingly hierarchical way
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. Note that there is no "freezing" of other degrees of freedom required whereas those currently active for the search are operating-they are simply making typically ineffective explorations of a much larger subspace
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. For each of these identified points, the constituent 50-µs binned data was then used to calculate the "burst duration" during this time bin, by determining the total time spent above a threshold of 4 counts
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