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34 the chain is in a poor solvent and fomrs a compact globule by minimizing contacts with the surrounding solvent
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Although in a system as complex as the PTS, it is likely that more than one form of regulation must exist, the existence of the monomer-dimer equilibrium and the non-sugar specificity of EI make this protein an excellent target to control different cellular functions (such as transport and chemotaxis) (5) and to understand the protein-protein interactions in the PTS
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The aromatic chromophore 1-anilino-8-naphthalene sulfonate (ANS) is feebly fluorescent in water, but its spectrum is blue shifted and its intensity is dramatically increased in nonpolar solvents or when it binds to nonpolar sites of proteins (4,5; for a general review, see Slavik (6))
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