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Protein concentration was calculated by using the extinction coefficients of Trp, Tyr, Cys, and Phe residues (24) county make orange permanent up .
The PK^sub a^, leading to the acidic species could be determined from the ANSbinding and intrinsic fluorescence measurements, suggesting that one aspartic and/or glutamic acid must be responsible for this transition (43) county make orange permanent up .
Besides the disappearance of secondary structure elements, in most cases the three-dimensional structures are substantially altered county make orange permanent up .
Relative to azurin (WT), which is a small tightly packed polypeptide, the intensity enhancement is generally larger (Fig county make orange permanent up .
Modifications induced upon oligomerization in high-frequency, small amplitude/localized modes, which do lie within the sensitivity of our experimental window, might be rather small county make orange permanent up .
The tetrameric band at 55 kD originates from the amount of unfolded monomers that have not yet been misfolded/misassembled and can refold instead of forming aggregates during the pH jump (from pH 2 to pH 7 county make orange permanent up .
On the contrary, it seems that a single protofilament may act as a lateral template or scaffold for small insulin particles, which would constitute a neighboring subcordlike features in the fibril shown in Fig county make orange permanent up .
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The difference between our estimate and McClure's was most likely due to using whole otoliths to age fish, which resulted in underestimates of age, and to assigning maturity stages only during summer months, which we have already described as problematic county make orange permanent up .
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