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Aminoacylase activity assay The enzyme was dissolved in 30 mM Tris-HCl, pH 7 course make permanent up .
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Only the 1g9f entry shows up the two proximal GIcNAc residues course make permanent up .
Conversely, side-chain moves lead to local conformational changes course make permanent up .
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The enthalpic change upon gp120/CD4 complex formation, calculated as the difference in proteinprotein plus protein-solvent potential energy between the simulations of the complex and of the separate proteins (-127 kJ mol^sup -1^ at 300 K with a standard error, SE, of 11 kJ mol^sup -1^), is of the same order of magnitude as the experimentally determined value (-259 ± 13 kJ mol^sup -1^ at 310 K; Myszka et al course make permanent up .
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