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In contrast PPA shows already in the native state a rather red-shifted emission spectrum (λ^sub max^ = 343 nm) which first decreases in intensity upon heating
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In both models the N-terminal domain of one CaM faces the C-terminal domain of the other CaM, creating a hydrophobic tunnel where SEF2-1mp is trapped
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Deriving the activation barriers assuming a two-state exchange is likely to be a significant simplification, as the conformational landscape may be much more complex
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A further aspect of nonequilibrium behavior exhibited by solutions of proteins with short-ranged attractive interactions is metastable liquid-liquid separation (11,12)
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Time resolved fluorescence anisotropy of extrinsic probes as a measure of protein aggregationFluorescence lifetime data from ANS, Bis-ANS, DCVJ, and ThT were recorded from the probes bound to both native and aggregated TTR (Fig
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