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. This is similar to previous observations with another FAD-dependent enzyme, pyruvate oxidase (Risse et al
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. In the intermediate q regime, proteins in the EV limit show scale-invariant, sequence-independent behavior wherein properties such as chain size and internal distances follow well-defined power laws
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. EI^sup sc^ was a well-folded turner that could be heat-denatured in a single sigmoidal transition, and thus it followed the second mechanism proposed
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. However, upon unfolding transitions generally distinct shifts of the emission spectra are reported (see for example (4,9-14))
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. The binding of soman to HuBChE via a single covalent bond (plus a salt bridge between a negatively charged oxygen on the phosphoryl moiety, and the protonated imidazole ring of His-438) may explain why it does not noticeably affect global HuBChE molecular dynamics
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. Pyrene excimer fluorescence as a proximity probe for investigation of residual structure in the unfolded state of human carbonic anhydrase II
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. Circular dichroism spectroscopy and light scatteringCircular dichroism (CD) and light-scattering data were recorded using AVIV-215 spectrometer (AVIV Biomedical, Lakewood, NJ) with fluorescence accessory and Pelletier temperature control
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. 7 mm BL, at a relatively large size range compared to that for Parapercis spp
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. Electrical Properties of Biopolymers and Membrane
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. The synchronous plot in Fig
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. Reference deleted in proof
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.5 mM PEP at pH 7
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. In proteins with more complex suctures, other candidates suggest themselves, such as the orientation of helices with respect to β-sheets with which they are in contact in α/β proteins, and the relative orientations of β-turns and partially-folded β-sheets in all-β elements
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. The use of solvent dye instead of the pigment may warrant investigation
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.Calculated from data pooled from 1996 through 1998
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