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As a zinc-dependent enzyme, ACYI is quite stable against heat-induced denaturation and can be regarded as a thermostable enzyme with an optimal temperature for activity of ~65°C
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The intensities of the bands at different times were measured by densitometry
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Study of the "molten globule" intermediate state in protein folding by a hydrophobic fluorescent probe
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1 B shows ^sup 1^H MR spectra obtained from these voxels, exhibiting notable differences: In the nucleus, the nonwater signal was very small, whereas large peaks typical of triglycerides could be observed in the animal and vegetal cytoplasm-they represent methine, the glycerol backbone, methylene, and methyl residues, as indicated in the figure
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In considering data obtained by x-ray crystallography, Frauenfelder and Petsko (11) found that binding of oxygen was accompanied by a reduction in B-factors of residues close to the heme pocket in myoglobin
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Dilatational and shear elasticity of gel-like protein layers on air/ water interface
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Once protein unfolding reached completion in the first jump, the sample was cooled and incubated at 25°C for 1000 s, which was sufficient for completion of the helical refolding (as indicated by flattening of the Θ^sub 222^(t)) but not for the disk reconstitution (as indicated by DSC, EM, and gel electrophoresis)
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All are addressed in detail fit Tharp & Young On Ice Cream technical short courses
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Because the tip approaching the surface induces a shift of AFM cantilevers' resonance frequency, the drive frequency, which has to be adjusted before engagement of the oscillating probe's first surface contact, is reduced until the vibrational amplitude reaches 95% of the maximum value
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Financial support from the Deutsche Forschungsgemeinschaft and the Fonds der Chemischen Industrie and the COST D30 action "High Pressure Tuning of Biochemical Processes: Protein Dynamics and Aggregation" is gratefully acknowledged
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The magnetite crystals are believed to have formed from the thermal decomposition of the siderite crystals in the reaction 3FeCO^sub 3^ -[arrow right] Fe^sub 3^O^sub 4^ + 2CO^sub 2^ + CO
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The important factors identified by Akki et al
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With pharmacies now dispensing every day in 3,048 Wal-Mart Supercenters and discount stores, 404 Sam s Clubs, 83 Neighborhood Markets and four health clinics, Wal-Mart is now the industry's fourth-largest pharmacy retailer, behind only Walgreens, CVS and Rite Aid
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"The Wal-Mart pharmacist, in many ways, is like a health ambassador to the rest of the store
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Pharmacy sales grew 20 percent last year to more than $10 billion, while OTC sales surged to $8 billion
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"If he's not successful in opening a gaming operation in Detroit, Barden hasn't ruled out the possibility of moving his headquarters out of the Motor City
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He reported that men were more competitive than women, and suggested that men are intrinsically motivated to express their competitive superiority over others
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Considerable educational efforts are made each fall to assure that college students are knowledgeable about the dangers of alcohol abuse and drug addiction
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50 caliber machine gun mounted on the rear cargo ramp has been one of the recent HX-21 validations
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The results of this experiment suggest that past observations of deviations from optimal strategies (e
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