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. Considering the commercial importance of aminoacylase, the results herein might facilitate the optimization of the conditions for the industrial use of ACYI
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. coelicolor, first, to understand in depth the mechanism of the PTS; and, second, to provide new clues on the protein folding problem
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. The effect of protein concentration and heating rates It is well known that the kinetics and the extent of aggregation often depend not only on heating rates and temperatures but also on protein concentration (9,35)
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. The interaction was not significant, F (1,140) =
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- ) In terms of weight, the A7 is the heavier of the two at 6
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. Here we applied this method to analyze delta and gamma dispersions for protein solutions
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. Phase diagram for acidic conformational states of apomyoglobin
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. Previous studies have indicated that the zinc ion is essential for the stabilization of the active site conformation as well as the protein structure (18,22-24)
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. Phase diagram analysis of IR and fluorescence data The phase diagram analysis, which is a sensitive tool to detect folding intermediates, was carried out as described previously (35)
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. For large x, entropy opposes stretching of the chain beyond its average value of R^sub e^
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.42 kcal mol^sup -1^) and hydrophobic contacts (ΔΔG = + 4
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. Although the three-dimensional structure of these α-amylases show up rather similar, the corresponding stabilities differ significantly (16,17)
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. This is particularly intriguing, since its three-dimensional structure reveals that the active site of AChE, which, like other serine hydrolases, features a Ser-His-Glu catalytic triad, is buried at the bottom of a deep and narrow gorge lined by aromatic residues (26)
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.To further demonstrate the use of fluorescence anisotropy decay, we probed TTR aggregation kinetics using time-resolved anisotropy
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.The "diffusion-collision" approach, on the other hand, is supported by strong experimental evidence that folding rates are controlled by the rate of diffusion of pieces of the open coil in their search for favorable contacts, rather than a driven collapse along some continuous energy surface (Jacob et al
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