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. Experiments conducted with copper-free azurin from Pseudomonas aeruginosa and mutants I7S, F110S, and C3A/C26A correlate the magnitude of the ice-induced perturbation, as inferred from the extent of ANS binding, to the plasticity of the globular fold, increasing with less stable globular folds as well as when the flexibility of the macromolecule is enhanced
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. The backbone atoms (N, Cα, and C') of the most stable structural elements of both proteins, the first α-helix of gp120 (residues 17-31), and part of the β-sheet of CD4 consisting of residues 26-30 and 82-86 (see Fig
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. Inquiries concerning nonexclusive or exclusive license for its commercial development should be addressed to the Patent Counsel, Johnson Space Center, (281) 483-0837
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