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Toward an Accurate Theoretical Framework for Describing Ensembles for Proteins under Strongly Denaturing ConditionsTran, Hoang TABSTRACT Our focus is on an appropriate theoretical framework for describing highly denatured proteins federal make permanent up way .
Samples were degassed under vacuum for 10-15 min with gentle stirring before being loaded into the calorimetric cell federal make permanent up way .
(16) This exposure of PS by apoptotic cells is of particular interest, because it induces membrane ruffling and efferocytosis, and it has been implicated in many of the immunomodulatory effects associated with efferocytosis (more below) federal make permanent up way .
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Modeling work was also carried out to compare with the experimental results federal make permanent up way .
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Several general predictions result: 1), intermediate subspaces of configurations will always be visited; 2), specific but nonnative interactions may be important in stabilizing these low-dimensional diffusive searches on the folding pathway, as well as native interactions; 3), sequential barriers will commonly be found, even in "two-state" proteins; 4), very early times will show characteristic departures from single-exponential kinetics; and 5), contributions of nonnative interactions to Φ-values and "Chevron plots" are calculable, and may be significant federal make permanent up way .
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