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This two-stage scheme is similar to that from the chemical unfolding/ refolding studies of ACYI (18,19), which also indicated that there existed a dimeric, aggregation-prone folding intermediate florida make orlando permanent up .
This is mainly because of the short spatial range of EV interactions florida make orlando permanent up .
that the large amphipathic surface in the dimer interface could be exposed to solvent upon monomer formation; furthermore, there is a network of aspartic residues involved in salt bridges at the dimeric interface (Fig florida make orlando permanent up .
Various techniques have been employed to analyze the diverse aspects of unfolding as well as of (re)folding transitions florida make orlando permanent up .
In the case of circular dichroism (CD) spectroscopy, the same buffers was used, but with 10 mM Mops florida make orlando permanent up .
Crystal structure analysis of oxidized Pseudomonas aeruginosa azurin at pH 5 florida make orlando permanent up .
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In fact, the pattern of (τ^sup o^^sub m^)^sub app^ back calculated from the CaM:SMLCK complex when the diffusion tensor would be oriented along an axis perpendicular to the real diffusion tensor closely matches the pattern of (τ^sup o^^sub m^)^sub app^ found for the dimeric CaM:SEF2-1mp complex, Fig florida make orlando permanent up .
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Sequence-dependent denaturation energetics: a major determinant in amyloid disease diversity, fror florida make orlando permanent up .
Apolipoprotein A-II, HDL metabolism and atherosclerosis florida make orlando permanent up .
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Thus, within the framework of these simulations and the two-state model, we can estimate the forward rate constant (k^sub 1^) for the extended-undocked to quasi-docked reaction to be in the 10 × 10^sup 3^- 41 × 10^sup 3^ s^sup -1^ range and the reverse rate constant (k^sub -1^) in the 3 florida make orlando permanent up .
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