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0 The scan rates were 30 K/h
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Fluorescence spectroscopy Intrinsic fluorescence was measured using a RF-1501 rluorospectrometer (Shimadzu, Duisburg, Germany) and a LS55 luminescence spectrometer (Perkin-Elmer, Rodgau, Germany)
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In the case of multitryptophan proteins, tyrosine fluorescence emission is generally not visible, mainly due to much larger absorption coefficients and quantum yields of the tryptophans, and at least for folded states because of distinct Förster energy transfer from tyrosines to tryptophans (3,14)
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3 this behavior is shown for BAA
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2% collagenase (Sigma, type I, St
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laevis embryos are ~1 mm in diameter, oocytes up to the 8-cell or 16-cell stages would, under optimal conditions, be observable at single-cell resolution with our (180 µm)^sup 3^ voxel size
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Initiation of oligomerization was performed through addition of 100 mM NaCl from a 4 M stock solution to 0
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An approach by reassembly techniques
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Three mechanisms of LLPS can be considered: nucleation and growth of the polymer-rich phase (globular microporous structure), spinodal decomposition (fibrillar microporous structure), and nucleation and growth of the solvent-rich phase (cell- tunnel microporous structure)
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Similarly, Nichol and Pikitch (1994) ob-served darkblotched rockfish undergoing an "immature cycling" and even assigned these fish a maturity stage
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3 B) as described in the Materials and Methods section
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