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Evidence for the existence of an unfolding intermediate state for aminoacylase during denaturation in guanidine solutions gilbert make melissa mineral up .
1 A, open squares)', 2), the ANS-binding experiments (Fig gilbert make melissa mineral up .
Although a quantitative relation between aggregation and reduction of the unfolding induced red shifts cannot be given, our data indicate that protein aggregation contributes significantly to smaller magnitudes of red shifts as observed during thermal unfolding gilbert make melissa mineral up .
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Establishing quantitative relationships between thermal fluctuations and functional motions is challenging, taking into consideration the vast variety of protein functions (synthesis, catalysis, electron- and proton transfer, etc gilbert make melissa mineral up .
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