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Thus, the value for K^sub SV^ will depend on the particular average intrinsic fluorescence lifetime of the protein, and may be higher than the value for free Trp givenchy irresistible perfume very woman .
RESULTS AND DISCUSSION The thermal dependence of ACYI activity was determined by measuring the catalytic activity of the samples equilibrated at given temperatures tor 30 min givenchy irresistible perfume very woman .
The accuracy of these ensembles is judged by matching the structural characteristics of the simulated ensembles to those predicted by field theories givenchy irresistible perfume very woman .
Both the Flory random-coil model and field theories agree that all sequence-specific biases are strictly local and that denatured-state ensembles show significant conformational heterogeneity givenchy irresistible perfume very woman .
The fluorescence of tryptophan residues is very sensitive to the local environment givenchy irresistible perfume very woman .
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The remaining drop was blotted dry using a filter paper givenchy irresistible perfume very woman .
6 and Table 1) shows:i givenchy irresistible perfume very woman .
5 nm observed after sample exposure to high temperatures (lanes 5 and 6 in Fig givenchy irresistible perfume very woman .
We work out on example in more detail in the case of three-helix proteins givenchy irresistible perfume very woman .
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In this paper, thermally induced phase separation (TIPS) is used to overcome this difficulty givenchy irresistible perfume very woman .
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