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. NH^sub 2^-terminal sequence truncation decreases the stability of bovine rhodanese, minimally perturbs its crystal structure, and enhances interaction with GroEL under native conditions
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. Third, a slightly brown colored aqueous layer was dialyzed against water until the pH of the protein solution became 5
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. Hydrophobic hydration analysis on amino acid solution by the microwave dielectric method
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. The decrease in the content of Class I Trp fluorophores suggested that the intermediate had a loose tertiary structure, which allowed the penetration of water molecules into the hydrophohic interior
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. In a previous study from the laboratory, we reported that the monomer of gSBA has a hydrodynamic radius of ~3
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. MR images were acquired using a spin-echo sequence with simultaneous suppression of the lipid peaks between 0 and 3 ppm using three successive sinc3 pulses of 3 ms length and 2070 Hz bandwidth centered at 1
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. Alterations in ^sup 1^H NMR peak levels from intracellular lipids have been reported in various cell biological processes, such as cell activation, proliferation, and apoptosis (31)
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. 4 kDa in humans) reduces the anti-atherogenic effects of HDL and modulates HDL metabolism via diverse mechanisms
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. The DSC data recorded of apoA-1- and apoA-2-containing disks upon heating at 90 K/h show that the unfolding/ dissociation of apoA-1 is centered at 91°C, ~7°C higher than the apoA-2 transition (Fig
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. Several works implicated importance of particular amino acid residues for insulin aggregation; for example, the bovine insulin, diverging from the human protein at three amino acid positions (A8 Ala^sup Bov^/Thr^sup Hum^, A10 Val^sup Bov^/ Ile^sup Hum^, and B30 Ala^sup Bov^/Thr^sup Hu^), aggregates more easily in vitro (Nielsen et al
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. Our suggestion is that a discriminating design of key nonnative interactions may significantly speed the search for the native state
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. The bridging sheet of gp120 also plays a key role in the entropy change, as has been proposed experimentally (Myszka et al
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. We used carrier supplied by Vertex Image Inc
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. ACKNOWLEDGEMENTSWe gratefully acknowledge the support of the National Textile Center for this project
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