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In the present study, we have investigated the equilibrium unfolding of yeast glutathione reductase (GR) by guanidine hydrochloride (GdnHCl)
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Northampton, MA) by using a protein concentration of 1
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Quantitative NMR studies of high molecular weight proteins: application to domain orientation and ligand binding in the 723 residue enzyme malate synlhase G
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Before comparing the behavior of individual proteins it is convenient to divide the group into two classes: one (β-gal, α-amylase, and ALD), which binds ANS essentially only in the frozen state, and the rest, which bind a fair amount of ANS also in liquid solution (4,5,28)
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For example, at 20 µm ANS where binding sites are fully saturated in solution and the intensity has reached its maximum value, the corresponding intensity in ice is merely 3%-5% of the value in the liquid water
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This monomer-dimer equilibrium is dependent on ionic strength and protein concentration
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Data fitting suggests that, in contrast to the double-exponential kinetics in the first jump (τ^sub fast^ = 190 s, τ^sub slow^ = 1630 s), the data in the second jump are well approximated by a single exponent with a relaxation time τ = 190 s, which is similar to τ^sub fast^
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Since overlap between blocks can reduce the standard deviation and hence the standard error (Fig
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Particles in the desired range (5 to 20 µm) were produced for EVA 9% and EVA 28%
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[FIGURE 1 OMITTED]The oldest eggs of P
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INTRODUCTIONRibonucleic acid (RNA) folding represents a critical step toward the generation of functional three-dimensional structures of RNA molecules
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