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. No changes in anisotropy were observed up to 2 M GdnHCl
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. A dominance of hydrophobic interactions was observed over hydrogen-bonding interactions
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. Reassessing random-coil statistics in unfolded proteins
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. 5 mM PEP and 5 mM Mg^sup 2+^), and every digestion was repeated three times
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. We hypothesize that this compensatory effect could be due to a change in the coordination sphere of the Mg^sup 2+^ by directly involving the phosphoryl group of the PEP moiety, as it occurs in a member of the pyruvate dikinase family (57)
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. In PEG 10000, the concentrations of salts required to collapse DNA were about half those with PEG 3000
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. The consequent mutual but general attraction of the helices for each other permits the final diffusive search for the native state to take place in a two-dimensional space, rather than the full five-dimensional search configuration space of all the diffusive degrees of freedom available to the protein once the secondary structure has formed
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. Effect of transforming growth factor-beta 1 (TGF-beta 1) on matrix synthesis by monolayer cultures of rabbit articular chondrocytes during the dedifferentiation process
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. The quaternary structure of GR in the presence of GdnHCl was also investigated by size-exclusion liquid chromatography
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. The fully deuterated samples were obtained by incubating the protein solutions in a water bath at 50°C for 15 min, and then the samples were cooled to room temperature and lyophilized
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. The activity of EI is regulated by a monomer-dimer equilibrium, where the autophosphorylation requires the dimeric species (2-4)
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. Then, one is tempted to ask, is there any relationship among the different functions and structures of the PTS proteins on one side, and the thennodynamic parameters and folding properties on the other? To answer this question, it is necessary to describe the conformational preferences of proteins, where studies of different members of the family have been described
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.1°C/min) gives rise to less pronounced aggregation during the thermal unfolding transition as compared to higher heating rates; and (2), the lowest protein concentration (7 µg/ml) reveals a less-pronounced aggregation also for a higher heating rate (1°C/min), whereas higher protein concentrations studied with the higher heating rate exhibit more pronounced aggregation
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. These intensity profiles are reminiscent of binding curves except for assuming a slightly sigmoidal shape due to a more gradual increase in the signal in the low concentration range
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. As a result, the small size of a hydrated cation provides effective interaction with DNA due to higher Coulomb electrostatic potential (Rouzina and Bloomfield, 1996) and the possibility of being incorporated into the DNA minor groove (Bartenev et al
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. X-ray microanalysis of HeLa S3 cells
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