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. 0) with 1-mM acetylthiocholine iodide as substrate
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. The influence of inhibitor-binding on global enzyme molecular dynamicsAlthough several studies using different techniques have shown a modification of global protein molecular dynamics on an Ångström-nanosecond scale upon inhibitor-binding, we were not able to detect any significant differences between the native and the inhibited HuBChE, at comparable degrees of hydration, up to ~334 K (61°C)
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. Equation 2 implies that when contact angle θ^sub c^ increases, the surface tension force moves toward negatives due to the cosine value of θ^sub c^, resulting in the negative wetting force
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. Early measurements (42) showed BLG has a dipole moment of a sufficient magnitude to generate significant attractive dipole-dipole interactions which are predicted to be screened by increasing ionic strength at the concentrations relevant to this study (40,43)
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. The spectra obtained upon Trp excitation (290 nm) are shown in Fig
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. For DNS-PE-labeled membranes, the labeled lipid was added to the chloroform lipid solution before drying
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. , 2003) and occurs above 50°C
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. BURGERIndiana State UniversityDONNA DAHLGRENIndiana University SoutheastCHRISTINE D
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