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Second, the heat-unfolding curve of the apoA-2:DMPC disks is sigmoidal and is shifted to higher temperatures as compared to that of free protein; thus the unfolding of free apoA-2 is complete at 80°C, well below the apparent melting temperature T^sub m^ = 84°C of the apoA-2:DMPC complexes observed at this scan rate (Fig hylyt dog shampoo .
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Effect of denaturant and Chevron plotsAs central a tool to the experimental elucidation of folding kinetics as the protein engineering extraction of Φ-values is the tracking of folding rate with denaturant concentration hylyt dog shampoo .
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Since the folding observed in the SL 4WJ ribozyme construct occurs in the absence of loop-loop interactions, the conformational change must be associated with the 4WJ helical junction element itself hylyt dog shampoo .
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