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On the reaction mechanism of yeast glutathione reductase
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This could be due to the fact that CD is reporting global changes in the secondary structure, and fluorescence is monitoring the changes in tertiary structure around fluorescent residues
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Given that early stage X
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Calculations (21) show that if a longer ranged attraction is added on to the AHS model, although the percolation line does not move, the effective stickiness varies such that the liquid-liquid coexistence line is no longer below the percolation line, a potential explanation for the absence of particle gelation
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Cross-linking of samples after incubation times >2 h showed a protein band that was unable to enter the gel, indicating formation of very large aggregates >1000 kD (data not shown)
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Previous studies of the pressure behavior of PLCδ showed that pressure increased the reaction rate of substrate hydrolysis of this enzyme (Rebecchi et al
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Thus, the size heterogeneity of the apoA-2:DMPC disks may not account for their multiphase unfolding kinetics
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