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. Unfolding was monitored by a variety of techniques, including intrinsic fluorescence emission, anisotropy and iodide quenching measurements, far-ultraviolet circular dichroism and thiol reactivity measurements
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. coli, EIN^sup ec^ (9), and the C-terminal domain of T
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. However, we cannot rule out that an alternative explanation to the titration curve is that the deprotonation of a histidine residue can be felt by a residue(s) far enough in the three-dimensional structure (see Fersht (45) and references therein) affecting its (their) environmcnt(s)
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. The newly frozen samples were then allowed to anneal for 12 h at -5°C, followed by a final equilibration of 2 h at the temperature selected for fluorescence measurements (-5 and -13°C)
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. 3, we tested internal motion models with either one (M1; τ^sub 1^ and S^sup 2^ fitting parameters) or two contributions (M2; τ^sub if^, S^sup 2^^sub f^, τ^sub is^ and S^sup 2^^sub s^ fitting parameters) to the internal motion
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. Two of the hinge regions are formed by the flexible part of the central helix of the CaM molecules, and the other two hinge regions are formed by the flexible dimerization interfaces
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. Reference spectra of conformational limits
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. The pressure behavior of membrane-bound CPM-PLCβ-Gβγ complexes were then studied in the presence of 50 nM Gα^sub s^(GDP) from atmospheric to 1000 kbar
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. RESULTSThermal transitions in lipid-free and in DMPC-bound apoA-2Far-UV CD data of lipid-free apoA-2 were recorded from 5 to 20 µg/ml protein solution in 10 mM Na phosphate, pH 7
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. In the combined trajectory analysis (Fig
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. Therefore, additional research is needed to determine if the current study's results can be replicated in a larger, more geographically diverse sample
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. The secondary structure of EI^sup sc^ upon binding was not largely affected (Table 4), but there were subtle changes in the stability of the protein (Table 3)
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. In contrast, heating to 100°C, i
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