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Thermodynamics of dcnaturant-induced unfolding of a protein that exhibits variable two-state denaturation
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3 B emphasizes that the value both of n and of the apparent dissociation constant are considerably affected by temperature
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Since the diameter of this gorge, at its narrowest point, is smaller than the cross-section of the quaternary group of ACh, substantial breathing motions of the whole AChE catalytic subunit are necessary to allow the substrate to penetrate to the active site
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This is believed to assist the target-specific deformation of the binding sites in CaM that is necessary for productive binding
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Akke and co-workers (38) have also found larger ΔH^sup #^ values for residues that are part of regular secondary structure and smaller values for loop residues, in their study on the C-terminal domain of CaM
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To enable conformational characterization of protein isolated from biological specimens such as samples from patients, noncovalent recognition using extrinsic dyes is necessary
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