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3), we used fluorescence quenching to further investigate the solvent accessibility of tryptophan residues upon unfolding
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The pretransitional structural changes involved the partial unfolding of the solvent-exposed β-sheet structures and the transformation of about half of the Class I Trp fluorophores to Class II
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that at 365 nm at different temperatures
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The oblong shape reflects the coupling between shape and size
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HPr^sup sc^, in contrast to the HPrs in Gram-negative bacteria and low-G + C Gram-positive bacteria (1), is not involved in general carbon regulation (20,21)
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Eur Respir J 2005; 25:447-454(8) Vandivier RW, Fadok VA, Hoffmann PR, et al
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In addition, other properties of the unfolded states, most probable structural compactness or simply differences in the conformational scrambling, also affect the magnitude of red shifts
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The excitation light, provided by a Cermax xenon lamp (LX150UV, ILC Technology, Sunnyvale, CA), is selected (λ^sub ex^ = 350 nm for ANS and 285 nm for azurin fluorescence, respectively, 6 nm bandwidth) by a 0
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3-5, the slope increases with increasing k^sup -1^^sub ex^ (see below; Fig
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This interaction perturbs the contacts that Gβγ makes with PLCβ, which causes enzyme activation
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This exemplifies that the correct criterion for thermodynamic reversibility is the absence of scan rate effects and hysteresis rather than the repeatability of a transition
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