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With each mutant azurin, a sizable fraction of binding sites exhibits affinities in the 1-2 mm range, corresponding to a dissociation constant 3-4 times lower than that of WT kochs barber chair .
laevis embryos are ~1 mm in diameter, oocytes up to the 8-cell or 16-cell stages would, under optimal conditions, be observable at single-cell resolution with our (180 µm)^sup 3^ voxel size kochs barber chair .
This latter observation has been ascribed elsewhere to contributions to the scattering signal from the hydration water protons, which are more flexible than protein protons in the temperature range above 260 K (44) kochs barber chair .
The contribution of vibrational entropy to molecular association kochs barber chair .
The N-H^sup N^ bond length was set to 1 kochs barber chair .
Circular dichroismFar ultraviolet circular dichroism (UV CD) spectra were recorded using a Jobin Yvon (Longjumeau, France) circular dichroism spectropolarimeter in the range 190-260 nm kochs barber chair .
ThT fluorescence at pH 1 kochs barber chair .
The insulin aggregation is promoted under conditions favoring partly destabilized monomers and dimers, such as low pH, high temperature, and contact with hydrophobic media (e kochs barber chair .
The importance of diffusive searches is unsurprising, since under biological conditions, all candidates for energetic interactions, including electrostatics, are locally screened to a few angstroms: much smaller than the dimensions over which sections of protein must move to find their native configurations kochs barber chair .
When the flat, diffusional degrees of freedom are projected away onto a reaction coordinate pair, such as R^sub g^ and [straight phi]^sub native^, then a folding funnel appears, and does so without the presence of any long-range forces kochs barber chair .
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These particles (D and E) appeared to be composed of dense material that was likely produced by spinodal decomposition (due to the high cooling rates) and later broken up during high-speed stirring kochs barber chair .
The spawning of plaice in the North Sea kochs barber chair .
Taxonomy of a bloom-forming cyanobacterial genus Microcystis kochs barber chair .
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That ASP system undermines a level playing field because it favors big purchasers of drugs that can squeeze rebates, volume discounts and other concessions from suppliers, said speaker Charles Oltman, national managed care administrator for Target Stores kochs barber chair .
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