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Thermodynamic data on the conformational stability of proteins can be obtained using several chemical or physical perturbants, including chaotropic agents (guanidine hydrochloride, urea), hydrostatic pressure, and temperature magnify make mirror up .
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(31) Third, Mitchell and colleagues (28) recently demonstrated that PSR-deficient fibroblasts ingest and respond to apoptotic cells normally magnify make mirror up .
J Cell Biol 2001; 155:649-660(22) Devitt A, Parker KG, Ogden CA, et al magnify make mirror up .
After eliminating the denaturing force the proteins are not able to fold back into their native (and functional) structure and the still unfolded proteins form (or just stay on with) aggregates magnify make mirror up .
The dependence of the ANS fluorescence intensity, in ice at - 13°C, on the starting ANS concentration in solution (10-200 µM) is shown in Fig magnify make mirror up .
Here we report a study of the backbone dynamics by ^sup 15^N-spin relaxation on the CaM dimer in complex with a dimeric peptide that mimics the CaM binding region of the bHLH transcription factor SEF2-1 magnify make mirror up .
This novel mode of target-specific binding, which neither belongs to lock-and-key nor induced-fit binding, is characterized by dimerization and continuous exchange between multiple flexible binding alternatives magnify make mirror up .
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It is however notable that the ThT molecule also responds to early oligomers (Fig magnify make mirror up .
We find that Gα(GDP) significantly decreased the volume change for the CPM-PLCβ-Gβγ association (Table 1) magnify make mirror up .
193 K/ min (from the apparent T^sub m^ = 84°C to 75°C) magnify make mirror up .
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