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. The concentration of ANS, as determined by absorbance at 370 nm, using ε^sub 370^ = 6800 M^sup -1^Cm^sup -1^ (in methanol), ranged between 10 and 450 µM although in most experiments the upper limit was 320 µM
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. This view is supported by both the signal amplitude and linewidth in the nucleus with time: Depending on the binding strength, bound molecules would give broad NMR peaks or no signal at all, and the diminazene signal is initially (after 1 h) much smaller than that of aceturate but, after 6 h, reaches ~½ of the aceturate amplitude
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